dapi satellite cell signal  (Cell Signaling Technology Inc)

Journal: Neuroscience

Article Title: MNK1 and MNK2 Expression in the Human Dorsal Root and Trigeminal Ganglion.

doi: 10.1016/j.neuroscience.2023.01.039

Figure Lengend Snippet: Fig. 1. Distribution of MKNK1 (MNK1) mRNA in human dorsal root ganglia. (A) Representative 20X images of human lumbar DRGs labeled with RNAscope in situ hybridization for MKNK1 (red) and SCN10A (blue) mRNAs and co-stained with DAPI (cyan). The fourth panel for each donor is a zoomed-in region demarcated by white boundaries in the 20X overlay image. Lipofuscin (globular structures) that autofluoresced in both channels and appear magenta in the overlay image were not analyzed as this is background signal that is present in all human nervous tissue. MKNK1 mRNA was expressed in neurons and non-neuronal cells. (B) Pie-charts showing the distribution of MKNK1 neuronal subpopulations in human DRG for each donor. (C) 92.4% of human DRG sensory neurons were positive for MKNK1. (D) Histogram with Gaussian distribution displaying the size profile of all MKNK1+ and SCN10A+ neurons in human DRG. (E) Percentage of MKNK1+ neurons that coexpressed SCN10A (blue bar), and the percentage of SCN10A+ neurons that coexpressed MKNK1 (green bar). Scale bars = 20X = 50 lm. Zoomed in panel = 20 lm.

Article Snippet: Total neuron counts for human samples were acquired by counting all of the probe-labeled neurons and all neurons that were clearly outlined by DAPI (satellite cell) signal and contained lipofuscin in the overlay image.

Techniques: Labeling, RNAscope, In Situ Hybridization, Staining

Journal: Neuroscience

Article Title: MNK1 and MNK2 Expression in the Human Dorsal Root and Trigeminal Ganglion.

doi: 10.1016/j.neuroscience.2023.01.039

Figure Lengend Snippet: Fig. 2. Distribution of MKNK2 (MNK2) mRNA in human dorsal root ganglia. (A) Representative 20X images of human lumbar DRGs labeled with RNAscope in situ hybridization for MKNK2 (red) and SCN10A (blue) mRNAs and co-stained with DAPI (cyan). The fourth panel for each donor is a zoomed-in region demarcated by white boundaries in the 20X overlay image. Lipofuscin (globular structures) that autofluoresced in both channels and appear magenta in the overlay image were not analyzed as this is background signal that is present in all human nervous tissue. MKNK2 mRNA was expressed in neurons and non-neuronal cells. (B) Pie-charts showing the distribution of MKNK2 neuronal subpopulations in human DRG for each donor. (C) 92.5% of human DRG sensory neurons were positive for MKNK2. (D) Histogram with Gaussian distribution displaying the size profile of all MKNK2+ and SCN10A+ neurons in human DRG. (E) Percentage of MKNK2+ neurons that coexpressed SCN10A (blue bar), and the percentage of SCN10A+ neurons that coexpressed MKNK2 (green bar). Scale bars = 20X = 50 lm. Zoomed in panel = 20 lm.

Article Snippet: Total neuron counts for human samples were acquired by counting all of the probe-labeled neurons and all neurons that were clearly outlined by DAPI (satellite cell) signal and contained lipofuscin in the overlay image.

Techniques: Labeling, RNAscope, In Situ Hybridization, Staining

Journal: Neuroscience

Article Title: MNK1 and MNK2 Expression in the Human Dorsal Root and Trigeminal Ganglion.

doi: 10.1016/j.neuroscience.2023.01.039

Figure Lengend Snippet: Fig. 3. Distribution of MKNK1 (MNK1) mRNA in human trigeminal ganglia. (A) Representative 20X images of human TGs labeled with RNAscope in situ hybridization for MKNK1 (red) and SCN10A (blue) mRNAs and co-stained with DAPI (cyan). The fourth panel for each donor is a zoomed-in region demarcated by white boundaries in the 20X overlay image. Lipofuscin (globular structures) were not analyzed as this is background signal that is present in all human nervous tissue. MKNK1 mRNA was expressed in neurons and non-neuronal cells. (B) Pie-charts showing the distribution of MKNK1 neuronal subpopulations in human TG for each donor. (C) 100% of human TG sensory neurons were positive for MKNK1. (D) Histogram with Gaussian distribution displaying the size profile of all MKNK1+ and SCN10A+ neurons in human TG. (E) Percentage of MKNK1+ neurons that coexpressed SCN10A (blue bar), and the percentage of SCN10A+ neurons that coexpressed MKNK1 (green bar). Scale bars = 20X = 50 lm. Zoomed in panel = 20 lm.

Article Snippet: Total neuron counts for human samples were acquired by counting all of the probe-labeled neurons and all neurons that were clearly outlined by DAPI (satellite cell) signal and contained lipofuscin in the overlay image.

Techniques: Labeling, RNAscope, In Situ Hybridization, Staining

Journal: Neuroscience

Article Title: MNK1 and MNK2 Expression in the Human Dorsal Root and Trigeminal Ganglion.

doi: 10.1016/j.neuroscience.2023.01.039

Figure Lengend Snippet: Fig. 4. Distribution of MKNK2 (MNK2) mRNA in human trigeminal ganglia. (A) Representative 20X images of human TGs labeled with RNAscope in situ hybridization for MKNK2 (red) and SCN10A (blue) mRNAs and co-stained with DAPI (cyan). The fourth panel for each donor is a zoomed-in region demarcated by white boundaries in the 20X overlay image. Lipofuscin (globular structures) were not analyzed as this is background signal that is present in all human nervous tissue. MKNK2 mRNA was expressed in neurons and non-neuronal cells. (B) Pie-charts showing the distribution of MKNK2 neuronal subpopulations in human TG for each donor. (C) 100% of human TG sensory neurons were positive for MKNK2. (D) Histogram with Gaussian distribution displaying the size profile of all MKNK2+ and SCN10A+ neurons in human TG. (E) Percentage of MKNK2+ neurons that coexpressed SCN10A (blue bar), and the percentage of SCN10A+ neurons that coexpressed MKNK2 (green bar). Scale bars = 20X = 50 lm. Zoomed in panel = 20 lm.

Article Snippet: Total neuron counts for human samples were acquired by counting all of the probe-labeled neurons and all neurons that were clearly outlined by DAPI (satellite cell) signal and contained lipofuscin in the overlay image.

Techniques: Labeling, RNAscope, In Situ Hybridization, Staining